Generator

Part:BBa_K4475005:Design

Designed by: Andrew Taylor   Group: iGEM22_FSU   (2022-07-18)


CTB1 Inducible Protein Generator Yeast


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 3974
    Illegal BamHI site found at 3883
    Illegal BamHI site found at 6056
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 6563
    Illegal AgeI site found at 70
    Illegal AgeI site found at 4637
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The native coding sequence was trimmed for exon sequences only so that a functional protein would be directly synthesized with minimal gene regulation considerations. The sequence was also codon optimized for yeast and tagged with an N-terminus HA tag.


Source

Genome of Cercospora Beticola

References